Welcome Break has reopened Fleet Services South at junction4a/5 on the M3 following a £20m investment project.
The new service station replaces the temporary structure that was installed following a major fire in 2016, when the building was destroyed.
The service station houses major high street brands, such as Starbucks, Little Waitrose and KFC as well as a new Pizza Express restaurant, which has seating for 166 customers, together with an outside seating area.
A total of 150 new jobs have been created at the site.
Welcome Break CEO, Robbie Bell (left), said: "We hope our customers will be impressed with the building itself, as well as the wide range of choice of high street brands."